Molecular Genetics
The Network has technical capacities to carry out analyses with an emphasis on genetics and functional genomics to identify, characterize, select, and improve genetic resources of interest (plants, animals, and microorganisms) to meet the specific demands of the agricultural sector.
This Network of Laboratories has three specialized areas:
- PrePCR: Biological material processing area for the extraction and quantification of DNA and RNA from viruses, bacteria, fungi, plant tissue, and animal tissue.
- PCR (Polymerase Chain Reaction): DNA fragments from regions of interest are obtained in this area. It is equipped with high-efficiency end-point and real-time thermocyclers.
- Post-PCR: This is the area where PCR fragment visualization and sequencing processes are carried out. It has spaces for agarose and polyacrylamide gel preparation and capillary electrophoresis (Sanger sequencing). Additionally, high-throughput methodologies are performed, such as large-scale genotyping with array chips and next-generation sequencing (NGS), whole-genome DNA sequencing, resequencing, and RNA sequencing (transcriptomics).
The Network of Molecular Genetics Laboratories is found in four research centers throughout the national territory.
Available Services
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Large-scale genotyping with array chips for animal and plant species
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Sequencing of fragments (Sanger).
| Analysis | Description |
|---|---|
| Molecular characterization |
Molecular characterization of the genetic resources of interest in:
|
| Molecular diagnostic | Identification and characterization of diseases of economic interest in plants and animals. |
| RNA seq studies | Transcriptomics and gene expression |
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